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Caine Veterinary Teaching Center
1020 E. Homedale Rd.
Caldwell, ID 83607
Ph: 208.454.8657
Fax: 208.454.8659


Website suggestions:
cvtc@uidaho.edu


 
Caine Veterinary Teaching Center
 

Laboratory Services

The Caine Center provides a number of laboratory services including Bacteriology, Virology, Histopathology and Clinical Pathology. Below is a list of tests available from each laboratory, as well as the required sample types and approximate testing times.

If you are not sure which tests to request, or cannot find the desired test on the list, please contact us at CVTC: (208) 454-8657 and ask to speak with a lab technician.

Samples requiring tests that are not performed at the Caine Center will be forwarded to outside laboratories for processing. Please call if the test you are requesting is not listed on this web site. You may want to deal directly with the outside laboratory. Shipping and handling as well as a referral fee will be charged in addition to the test charges.

Sample Submission to CVTC:

  • Whether you are shipping samples via USPS or hand-delivering to our office, please be sure to package samples so that no one handling the package is at risk for exposure to pathogenic organisms. Remember: all samples are suspect and must be packaged accordingly.
  • Avoid shipping samples over holidays and weekends. Most delivery services will be unable to get packages to CVTC, and we have limited staff and may not be available to accept samples.
  • Be sure items to be shipped are secure and in leak-proof containers, and include ice packs to keep samples cold up to 48 hours.
  • New Clients must complete a New Account Information Form and Laboratory Submission form please click here for download.

Clinical Pathology/Histopathology

Contacts: Amy Yacavace - James J. England, DVM

Virology/Serology

Contacts: Beth Mamer, PhD - James J. England, DVM

 

All personnel can be reached at (208) 454-8657.

Submission Guidelines (all labs)

Improperly collected samples may lead to longer processing times and erroneous diagnoses. Take care in the storage and shipping of the samples being submitted to the CVTC. The following are some guidelines to optimize the accuracy of the diagnosis. If you have any questions regarding sample collection, submission or testing, please call the Caine Center and speak with a technician: (208) 454-8657.
  1. All samples must be submitted with an Accession Form. New clients please use our New Client Account Form combined with our standard accession form. When completing any accession form please include:
    • Contact information for veterinarian and owner. Results will be reported to the clinician and/or owner as indicated on paperwork, except for antibiotic sensitivity results which must be reported to a veterinarian.
    • Species, breed, age, sex and ID of each animal.
    • Brief history including drug treatments.
    • List of samples being submitted and desired tests for each.
    • Place paperwork in a sealed container or zip lock bag, separate from samples.
    • Contact information for veterinarian and owner. Results will be reported to the linician and/or owner as indicated on paperwork, except for antibiotic sensitivity results which must be reported to a veterinarian.
  2. Samples must be submitted in clean, leak-proof containers.
    • Use appropriate containers for each sample, e.g. Whirl-Paks, fecal cups, milk tubes, etc. Do NOT submit liquid samples in bags. Gloves are not acceptable sample containers
    • Use leak-proof containers.
    • External surfaces of containers must be free of contamination by organic materials. Double bagging may be necessary to reduce risk of contamination.
    • If submitting a sample in a syringe (e.g. joint tap, etc.) the end of the syringe must be covered - use either syringe cap or covered needle.
  3. Clearly label all samples with the animal ID and the type of sample (lung, liver, tracheal swab, abscess aspirate, etc.) Outside container should be labeled with the owner, veterinarian and animal ID.
  4. If testing is to be done in multiple laboratories, divide the samples and label them for the appropriate laboratory and desired tests. Please provide adequate material for each lab. If you are not sure what testing might be needed, single, intact samples may be submitted for the technicians to distribute as needed.
  5. If submitting large numbers of samples, call the laboratory for scheduling.
  6. Samples submitted after 4:00 PM may be processed the following day.

Microbiology

Bacteriology/Mycology:

In our lab we are able to culture and identify common pathogenic bacteria and fungi from a variety of samples including tissues, swabs and aspirates, fecal material, etc. Testing often takes 3-5 days before meaningful results can be reported.

Samples:

  • Tissues for culture (lung, spleen, liver, kidney, etc.) should be at least 2 inches cubed (about the size of a fist) and include smooth membrane surface when possible. Fluids (including milk) for culture should be in sterile tubes and at least 2mL. Do not overfill tubes. Syringes should be capped.
  • Fecal and intestinal samples should be at least 3 grams.
  • Specimens for isolation of anaerobic pathogens including Clostridium spp. should be sent in an anaerobic transport system when available. Aspirates may be sent in a capped syringe as long as no air has been introduced. Larger tissue samples may be sent in high quality zipper bags or Whirl-Pak bags. It is best to avoid exposure to air as much as possible.
  • Bovine Trichomonas Samples For an official Idaho test, samples must arrive at the laboratory within 48 hours of collection and include an Idaho Trichomoniasis Test and Report Form. Forms must be signed by the veterinarian taking the samples. All samples should be individually labeled and kept cool during shipping. Use ice packs if needed. High temperatures will kill these protozoa.

Bacteriology/Mycology
Test Sample Types Approximate Time for Results
Abortion Screen for Sheep and Goats Placenta and or cotyledons, fetus, fetal tissues 5-10 days
Acid-fast Stain Feces, intestine, tissues 24 hours
Aerobic Culture (Standard) Any 3-5 days
Anaerobic Culture Tissue, aspirate, etc. NO SWABS 3-5 days
Anaerobic Identification Culture of anaerobic organism 24 hours*
Antibiotic Sensitivity (disk diffusion method)-Food Animal Antibiotic Panel-Companion Animal Antibiotic Panel-Mastitis Panel Culture of isolated organism 24-48 hours (does not include time for isolation and identification of organism).
Api® Identification Isolated organism 24-48 hours*
Blood Culture Blood culture bottles 1 week
Bulk Tank Analysis and Enumeration Milk from Bulk Tank 3-5 days
Campylobacter culture Fetus, Placenta, Feces 5 days
CL (Caseous lymphadenitis) Corynebacterium pseudotuberculosis Abscess, aspirate, swab, ask for standard aerobic culture 3-5 days
Chlamydia FA stain Abortion, eye swab, etc. 24-48 hours
Cryptosporidium (AF Stain) Feces, intestine, slide 24 hours
Enteric Culture for E. coli, Salmonella, etc. Feces, intestine, lymph nodes, etc. 3-5 days
Environmental Analysis and Enumeration Feed, bedding, soil, swab 5-10 days
Environmental Salmonella Culture Feed, bedding, soil 5-10 days
Fungal Culture Any Up to 3 weeks
Johne's Culture Feces, intestine, intestinal lymph nodes Up to 8-16 weeks
Listeria Culture Brain, spinal fluid, Milk, Fetus, Placenta Up to 12 weeks
Milk cultures Milk 3-5 days
Mycoplasma Culture (Mammalian or Avian culture) Milk, respiratory tissue, joints, swab, etc. 10 days
Neonatal Diarrhea Feces, intestine and/or lymph nodes 3-5 days
Salmonella Culture Any, including lung, liver, ileum, feces, mesenteric lymph nodes 3-5 days
Standard Aerobic Culture Any 3-5 days
Vibrio Culture (Campylobacter) Fetus, placenta, feces 5 days
Other Tests
Test Sample Types Approximate Time for Results
E. coli K99 Pili-Test Feces, intestinal content, culture of E. coli 24 hours
Salmonella Serogrouping Isolated Salmonella culture 24 hours
Somatic Cell Count Milk 24 hours
Trichomonas evaluation Diamonds media tubes or pouches - samples must reach the lab within 48 hours of collection. 7-8 days
Microscopy (Slides)
Test Sample Types Approximate Time for Results
FA: Chlamydia, Toxoplasma    
Acid-fast Stain Any, including slides 24 hours
Chlamydia FA Stain Any, including slides 24-48 hours
Clostridium FA Stain Any, including slides 24-48 hours
Cryptosporidiosis Feces, intestine, slides 24 hours
Darkfield Microscopy for Spirochetes including Leptospira Urine, kidney, intestinal- need to be tested within 1 hour of collection Same day
Gimenez Stain for Chlamydia, Rickettsia, Q Fever Any, including slides 24 hours
Gram Stain Any, including slides 24 hours
*Abortion Screens
Test Sample Types Approximate Time for Results
Ovine/Caprine Abortions Placenta, fetus, fetal tissues and/or cotyledons 5-10 days
FA Stains: Chlamydia, Toxoplasma Placenta, brain, lung, liver, kidney 48hrs
*Placenta is more important than fetus. If no placenta is available try to submit uterine exudate.    
**Bovine Large Abortions Greater than 15 lbs Necropsy and send samples Whole fetus under 15 lbs. Placenta or Cotyledons and tissues only on a larger fetus. (Brain, lung, heart, liver, kidney), Stomach content for Trichomonas testing 5-7 days
**Abortion serology is sent to WSU. Paired sera are recommended    
FA Stains: IBR, BVD, Neospora Placenta/Cotyledons, brain, lung, liver, kidney 48hrs
Ag Capture ELISA: BVD Skin from fetus and serum and ear notch from Dam 24-48hrs
Camelid Abortions Whole fetus including placenta  
FA Stains: BVD, Toxoplasma, Chlamydia Placenta/Cotyledons, brain, lung, liver, kidney 48hrs

Virology - Serology:

Samples:

  • Submit only fresh, unfixed samples (no Citrated or Heparinized blood, no Formalin fixed tissues).
  • Each sample must be clearly labeled with a readable animal ID#.
  • Tests are run at least once a week. Results are reported to the clinician/owner as noted on paperwork.
  • Sera may be used to test for BVDV in either pre-colostral calves or post-colostral cattle older than 3 months of age. This time frame is dependent on the amount and quality of colostrum received.
  • An ear notch biopsy sample can be submitted for any animal regardless of age to test for PI status.

Virology/Serology
Test Sample Types Approximate Time for Results
ELISA TESTS    
Bovine Virus Diarrhea (BVD) Ear notch, blood (Red-top or EDTA tube), tissue 24-48hrs
Bovine Leukemia Virus (BLV) Blood (Red-top tube) 24-48hrs
Johne's Blood (Red-top tube) 24-48hrs
Fluorescent Antibody Stains
Viral FA Stains
Test Sample Types Approximate Time for Results
Infectious Bovine Rhinotracheitis (IBR) Trachea, lung, trans tracheal swab 48hrs
Bovine Viral Diarrhea (BVD) Lung, liver, kidney, brain, blood (EDTA tube) 48hrs
Bovine Parainfluenza Type 3 (PI3) Lung, trans tracheal swab 48hrs
Bovine Respiratory Syncytial Virus (BRSV) Lung, trans tracheal swab 48hrs
Blue Tongue (BT) Blood (EDTA tube), lung 48hrs
Bovine Adenovirus Type (BAV1 & 3) Lung, trans tracheal swab, intestine 48hrs
Bovine Adenovirus Type 5 (BAV5) Lung, trans tracheal swab, intestine 48hrs
Bovine Corona Virus (BCV) Intestine, lung 48hrs
Transmissible Gastroenteritis (TGE) Intestine 48hrs
Porcine Parvovirus (PPV) Intestine 48hrs
Bacterial FA Stains
Test Sample Types Approximate Time for Results
Clostridium chauvoei Tissue (muscle) 48hrs
Clostridium novyi Tissue (liver) 48hrs
Clostridium septicum Tissue 48hrs
Clostridium sordelli Tissue 48hrs
Chlamydia psittaci and Chlamydia trachomatis (polyclonal) Tissue, eye swabs, joint sample 48hrs
Protozoa FA Stains
Test Sample Types Approximate Time for Results
Neospora caninum Tissue (brain, lung, liver, kidney, placenta) 48hrs
Toxoplasma gondii Brain, cerebrum and cerebellum, placenta, lung 48hrs
Darkfield Microscopy
Test Sample Types Approximate Time for Results
Leptospira Kidney, Urine <1hr from death Same day

Clinical Pathology and Histopathology:

Samples:

  • All samples must be submitted with the appropriate paperwork, identification numbers, and sample type clearly marked.
  • Tissue specimens for histopathology should be submitted fresh or in formalin (1 part tissue to 10 parts formalin).
  • Tissue pieces should be ¼ to ½ inch thick if possible. Remember interpretation will be adversely affected by autolyzed specimens.

Clinical Pathology
Test Sample Types Approximate Time for Results
Blood Gas Heparinized blood Same day
CBC EDTA Blood Same day
Chemistry Screen Serum from red or marble top tube Same day
Electrolytes Serum from red top, marble top or heparinized blood Same day
Heinz bodies EDTA Blood Same day
Plasma Protein EDTA or heparinized blood Same day
Reticulocytes EDTA Blood Same day
Total Protein Serum from red or marble top tube Same day
Urinalysis 10ml of urine Same day
Parasitology
Test Sample Types Approximate Time for Results
Flotation 5g of feces Same day
Sedimentation (for hepatica species) 5g of feces Same day
Baerman (for lungworm) 5g of feces Same day
Serology (AGID)
Test Sample Types Approximate Time for Results
Caprine Arthritis Encephalitis (CAE) 1ml of serum from red or marble top 48hrs
Ovine Progressive Pneumonia (OPP) 1ml of serum from red or marble top 48hrs
Blue Tongue (BT) 1ml of serum from red or marble top 48hrs
Histopathology
Test Sample Types Approximate Time for Results
Chronic Wasting Disease (CWD) Obex in formalin or Elk head  
Stains/Slides Tissue in formalin  
 
 
University of Idaho College of Agricultural and Life Sciences